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For such setae being stiffer if compared the substrate, they potentially show increased edge stress concentration. It is argued that discoidal setae, when softer if compared to the substrate, may show an advantageous peak-free interfacial stress distribution when being pulled off the substrate. This sexual dimorphism in attachment ability is explained by the presence of a specialized, discoidal seta type in males, which is not present in females. Whereas in females, the attachment ability was not affected by the substrate stiffness within the range of tested stiffness, males showed decreasing attachment ability with decreasing substrate stiffness. In order to test the effect of different substrate stiffness on the attachment ability of insects, we have performed friction experiments with female and male ladybird beetles Coccinella septempunctata on smooth silicone elastomer substrates of different stiffness, using a centrifugal force tester. However, the natural habitats of climbing animals may provide a variety of substrate stiffness ranging from rigid rock surfaces to soft, biofilm covered substrates. So far, the attachment ability of insects and also that of spiders and geckos has been tested on rigid substrates only. The function of these adhesive organs was shown to be affected by environmental conditions such as substrate roughness, chemistry, and ambient humidity. Many insects possess adhesive foot pads, which enable reliable attachment to diverse and unpredictable substrates. Sexual dimorphism in the attachment ability of the ladybird beetle Coccinella septempunctata on soft substrates
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